. 2022 Apr 27;13:875887. doi: 10.3389/fphar.2022.875887

TABLE 4.

Lymphoid populations (relative abundance) in the lung of SP-C^I73TGATA1^WT and SP-C^I73TGATA1^KO mice.

Lymphoid	Genotype	CTL (n=6)	7d (n=5)	14d (n=4)	42d (n=4)
Total Lymphocytes CD3⁺	SPC^I73TGATA1^WT	17.33 ± 1.36	9.71 ± 2.54 ^a	6.43 ± 1.51 ^a	20.97 ± 1.14 ^b ^, ^c
Total Lymphocytes CD3⁺	SPC^I73TGATA1^KO	12.51 ± 0.85	14.58 ± 3.08	15.27 ± 1.56	21.32 ± 1.26^a
T Cells CD3⁺CD4⁺	SPC^I73TGATA1^WT	9.50 ± 0.60	3.64 ± 0.87 ^a	2.29 ± 0.42 ^a	9.20 ± 0.55 ^b ^, ^c
T Cells CD3⁺CD4⁺	SPC^I73TGATA1^KO	4.96 ± 0.44	6.51 ± 1.48	7.90 ± 1.42	10.98 ± 0.91 ^a ^, ^b
T Cells CD3⁺CD8⁺	SPC^I73TGATA1^WT	0.89 ± 0.16	1.10 ± 0.28	0.22 ± 0.03	3.05 ± 1.20 ^a ^, ^c
T Cells CD3⁺CD8⁺	SPC^I73TGATA1^KO	1.77 ± 0.20	1.48 ± 0.23	0.85 ± 0.27	1.64 ± 0.17
Dendritic Cells CD4⁺CD103⁺	SPC^I73TGATA1^WT	0.23 ± 0.02	0.21 ± 0.04	0.92 ± 0.40	0.62 ± 0.14
Dendritic Cells CD4⁺CD103⁺	SPC^I73TGATA1^KO	0.10 ± 0.01	0.81 ± 0.41	0.73 ± 0.14	0.84 ± 0.07
Dendritic Cells CD8⁺CD103⁺	SPC^I73TGATA1^WT	3.36 ± 0.41	2.41 ± 0.80	0.42 ± 0.11 ^a	4.78 ± 0.09 ^b ^, ^c
Dendritic Cells CD8⁺CD103⁺	SPC^I73TGATA1^KO	1.87 ± 0.32	2.69 ± 0.58	1.28 ± 0.33	3.38 ± 0.39 ^c
Total B Cells B220⁺	SPC^I73TGATA1^WT	20.06 ± 2.95	7.38 ± 3.07 ^a	3.22 ± 1.15 ^a	18.64 ± 1.56 ^b ^, ^c
Total B Cells B220⁺	SPC^I73TGATA1^KO	28.35 ± 2.07	12.07 ± 4.08 ^a	9.42 ± 1.68 ^a	13.67 ± 1.18 ^a
B Cells B220⁺CX3CR1^-	SPC^I73TGATA1^WT	8.03 ± 1.60	2.06 ± 0.90 ^a	1.93 ± 0.59 ^a	7.49 ± 1.01 ^b ^, ^c
B Cells B220⁺CX3CR1^-	SPC^I73TGATA1^KO	8.17 ± 0.49	4.66 ± 1.93	1.71 ± 0.55 ^a	1.84 ± 0.17 ^a
B Cells B220⁺CX3CR1⁺	SPC^I73TGATA1^WT	12.03 ± 2.01	5.32 ± 2.19	1.29 ± 0.63 ^a	11.15 ± 0.70 ^c
B Cells B220⁺CX3CR1⁺	SPC^I73TGATA1^KO	20.18 ± 1.66	7.42 ± 2.27 ^a	5.47 ± 2.09 ^a	11.83 ± 1.07 ^a

Single cell suspensions of SP-C^I73TGATA1^WT and SP-C^I73TGATA1^KO lung digests was assessed by flow cytometry, enriched in viable CD45⁺ singlets, and analyzed following gating strategy described in the Methods section. Relative abundance (%) is presented as means ± SEM (N=4-6 mice/condition). Data were analyzed by two-way ANOVA, with Tukey post-hoc test.

Significant compared to controls (CTL).

Significant compared to 7 d group.

Significant compared to14 d group.