Figure 7.

(a and b) RT-qPCR and western blot analysis validated the idea that MBOAT2 is highly expressed in PDAC cells. (c and d) RT-qPCR confirmed the transfection efficiency of MBOAT2 in AsPC-1 and PANC-1 cells. (e–h) MTT and colony formation assays showed that MBOAT2 overexpression promotes PDAC cell proliferation, while MBOAT2 knockdown represses the proliferation. (i and j) Transwell assay was conducted to evaluate the migration ability of AsPC-1 and PANC-1 cells transfected with LV-MBOAT2 and LV-MBOAT2-RNAi. P values were assessed using two-tailed t-tests and ANOVA, followed by Dunnett's tests for multiple comparisons in (c)–(j). All data represent the means ± SD from three independent experiments (^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001; ^∗∗∗∗P < 0.0001).