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. 2022 Apr 27;605(7909):349–356. doi: 10.1038/s41586-022-04642-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — (a) White blood cell (WBC) count in 18-wk old mice. (b, c) Flow cytometric plots and quantification. (b) Spleen T (CD3⁺):B (B220⁺) cell ratio from 12-wk old kika mice. (c) Age-associated B cells (ABC, B220⁺ CD21⁻ CD23⁻ CD19^hi CD11c⁺) in blood from 18-wk-old kika mice. (d) Splenic marginal zone (MZ) B cells (CD19⁺ CD23⁻ CD21⁺) in 12-wk kika mice. (e) Circulating T follicular cells (Tfo, CD4⁺ CXCR5⁺ PD1^hi) and extrafollicular helper T cells (eT_H, CD4+ CXCR5⁻ PD1⁺ CXCR3⁺) in blood from 18-wk-old kika mice. (f) Plasmacytoid dendritic cellc (pDCs, CD3⁻ CD19⁻ MHCII⁺ CD11c⁺ CD11b^- CD8⁻ SiglecH⁺ BST2⁺) and pDC MFI of MHCII and SiglecH from 12-week-old kika mice. (g) Splenic germinal center B cells (GCB, CD19⁺ CD95⁺ BCL6⁺), ABC (B220⁺ CD21⁻ CXCR5⁻ CD19^hi CD11c⁺), T_FH (CD4⁺ CXCR5⁺ PD1^hi), eT_H (CD4+ CXCR5⁻ PD1⁺ CXCR3⁺) and plasma cells (PC, CD138⁺ CD98⁺) from 24-wk TLR7-deficient mice. (h, i) Splenic cell subsets from mixed bone marrow chimeric mice containing a 1:1 ratio of control Tlr7^+/+ CD45.1/Tlr7^+/+ CD45.2 or Tlr7^+/+ CD45.1/Tlr7^kik/kik CD45.2 bone marrow (h) and 100% mixed bone marrow (i) of each genotype. Subsets shown are CD4 effector (CD4⁺ FoxP3⁻ CD44⁺), MZ (CD19⁺ CD23⁻ CD21⁺), Treg (CD4⁺ FoxP3⁺) and CD45.1 to CD45.2 reconstitution ratio, 22-weeks post-reconstitution. (j) Autoantibodies to DNA and smRNP in serum from 100% chimeric mice model. (k) Splenic cell subsets from kika and Yaa mice. Bars represent medians and each dot a single mouse. These results are representative of one blood ADVIA analysis, two experiments for blood flow cytometry, four splenic phenotyping for kika mice and one for TLR7 deletion mice, and one experiment for chimera analysis. One-way ANOVA with Tukey (a–g, k); Two-way ANOVA (h); Mann-Whitney (i); Unpaired t-test (j); Exact p values are shown.