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. 2022 Apr 27;13:868377. doi: 10.3389/fmicb.2022.868377

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Copyright © 2022 Li, Chen, Dong, Liang, Guo, Chen, Huang, Liao and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Back mutation restored the interaction between gp85 and Tva. (A) Schematic representation of back mutation. (B) The interaction of A-gp85-flag wt, E-gp85-flag wt, and back mutant protein E/A-gp85-flag with Tva-HA-Fc. (C) DF-1 cells were incubated with A-gp85 wt, E-gp85 wt, and back mutant E/A-gp85 protein and subsequently infected with RCASBP (A)-EGFP. The cells were visualized under a fluorescence microscope and the percentage of GFP-positive cells (indicated with red color letters) was detected by FACS for blocking analysis.