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. 2022 May 11;13(5):449. doi: 10.1038/s41419-022-04866-x

Fig. 3. ER mimetics protect against OGD/R-induced microglial activation in HAPI cells, and potentiate OGD/R-induced angiogenesis in BMVECs.

Fig. 3

HAPI cells or BMVECs were pre-treated with ER mimetics, followed by OGD (2 h)/R (24 h) treatment. A The cell viability of HAPI cells was monitored by MTT assays. B The cell apoptosis of HAPI cells was monitored by Annexin-V/PI staining. C, D The mRNA or secretion levels of cytokines in HAPI cells were determined by qRT-PCR and ELISA assay, respectively. E The protein levels of target proteins in HAPI cells were determined by Western blotting. F The cell apoptosis of BMVECs was monitored by Annexin-V/PI staining. G The cell viability of BMVECs was monitored by MTT assays. H The angiogenesis of BMVECs was evaluated by tube formation assay. I The protein levels of target proteins in BMVECs were determined by Western blotting. β-actin acted as a loading control for Western blotting. *p < 0.05, **p < 0.01, and ***p < 0.001.