Skip to main content
. 2022 May 11;13(5):449. doi: 10.1038/s41419-022-04866-x

Fig. 4. Overexpression of SIRT6 alleviates OGD/R-induced microglial activation in HAPI cells, and potentiates OGD/R-induced angiogenesis in BMVECs.

Fig. 4

HAPI cells or BMVECs were infected with lentivirus for 48 h prior to OGD/R treatment. A The cell viability of HAPI cells was monitored by MTT assays. B The cell apoptosis of HAPI cells was monitored by Annexin-V/PI staining. C, D The mRNA or secretion levels of cytokines in HAPI cells were determined by qRT-PCR and ELISA assay, respectively. E The protein levels of target proteins in HAPI cells were determined by Western blotting. F The cell viability of BMVECs was monitored by MTT assays. G The cell apoptosis of BMVECs was monitored by Annexin-V/PI staining. H The angiogenesis of BMVECs was evaluated by tube formation assay. I The protein levels of target proteins in BMVECs were determined by Western blotting. β-actin acted as a loading control for Western blotting. *p < 0.05, **p < 0.01, and ***p < 0.001.