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. 2022 May 11;13(5):449. doi: 10.1038/s41419-022-04866-x

Fig. 5. SIRT6 suppresses TXNIP in HAPI cells and BMVECs via deacetylation of H3K9ac and H3K56ac.

Fig. 5

HAPI cells or BMVECs were infected with lentivirus for 48 h. A, B The mRNA and protein levels of TXNIP were determined by qRT-PCR and Western blotting, respectively. C Thioredoxin reductase activity was measured using commercial kit. D The protein levels of target proteins were determined by Western blotting. β-actin acted as a loading control for Western blotting. E, F The enrichment of SIRT6 at TXNIP promoter was determined by ChIP assay. Normal IgG served as a negative control. G The enrichments of total H3, H3K9ac, and H3K56ac at TXNIP promoter were determined by ChIP assay. *p < 0.05, **p < 0.01, and ***p < 0.001.