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. 2022 May 11;12:7747. doi: 10.1038/s41598-022-11770-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Area (A_cell) and its effect on the area normalized parameters (F_max/A_cell, E_max/A_cell,) presented on histograms with fits of lognormal distributions (A,C,E) and box charts (B,D,F). A significant difference was found in the A_cell between colorless cells and all other subpopulations (B). Also, the F_max/A_cell values between colorless cells, green and yellow cells were observed to be significantly different (D). E_max/A_cell has not shown any difference in their distribution (F).