Figure S3. Flow cytometry analysis of reactive oxygen species (ROS) production in HC (n = 18), PLWH_EC (n = 16), and PLWH_ART (n = 18).

Related to Fig 4. (A) Gating strategy for CD4⁺ T cells, CD8⁺ T cells, classical monocytes (CM), intermediate monocytes (IM), and non-classical monocytes (NCM) from total PBMCs. (B) Proportion of CD4⁺ and CD8⁺ T cells in HC, PLWH_EC, and PLWH_ART. (C) Proportion of CM, IM, and NCM in HC, PLWH_EC, and PLWH_ART. (D) UMAP shows the heat map distribution of ROS in HC, PLWH_EC, and PLWH_ART in lymphocytic and monocytic cell populations. (E) MFI of ROS in monocytic cell populations from PLWH_EC, short-term ART (<10 yr, n = 8), and long-term ART (>10 yr, n = 8). The histogram shows a representative sample from HC, PLWH_EC, and PLWH_ART exhibiting the median expression in each group. Statistical significance was determined using Mann–Whitney U test (P < 0.05 with * < 0.05, ** < 0.033, *** < 0.002) and represented with the median.