TABLE 3.

Amplification results (Ct values) of new Orien16S qPCR assay in specimens from human scrub typhus cases and mite pools from Chiloé Island.

Clinical samples
No.	Patient sex/age (years)	Sample type	Orien16S Cta value	References
1	Male/43	Eschar	31.76	(5, 7)
2	Male/56	Eschar	24.65	(5, 7)
3	Male/56	Buffy coat	35.62	(5, 7)
4	Male/25	Eschar	29.76	(5, 18)
5	Male/69	Eschar	25.65	(5, 7)
6	Male/69	Buffy coat	29.77	(5, 7)
7	Female/22	Eschar	27.38	(5, 7)
8	Male/25	Eschar	28.09	(5, 7)
9	Male/39	Eschar	28.76	(5, 7)
10	Male/39	Buffy coat	26.8	(5, 7)
11	Male/28	Eschar	31.58	(5, 7)
12	Male/28	Buffy coat	32.85	(5, 7)
13	Female/21	Eschar	25.99	(5, 7)
14	Male/20	Eschar	29.41	(17)
15	Male/17	Eschar	35.54	(17)
16	Male/55	Eschar	34.16	(4, 7)
17	Male/73	Eschar	30.97	(7)
18	Female/49	Eschar	27.12	(7)
19	Female/49	Buffy coat	35.12	(7)
20	Female/30	Eschar	24.35	(7)
21	Male/54	Eschar	25.12	(7)
22	Male/63	Eschar	26.28	(7)
23	Female/23	Eschar	28.4	(7)
24	Male/53	Eschar	23.45	(7)
25	Male/41	Eschar	24.55	(7)
26	Female/54	Eschar	26.82	(7)
27	Male/44	Eschar	29.39	(18)
28b	Male/62	Eschar	29.23	(16)
Mite samples
No.	Sample ID	Sample type	Orien16S Ct value	References
1	A olivacea/site4_1	Mite pool	33.48	(11)
2	A olivacea/site4_4	Mite pool	30.94	(11)
3	A sanborni/site4_1	Mite pool	33.14	(11)
4	A olivacea/site6_1	Mite pool	31.64	(11)

^aCt, cycle threshold. Ct value of 36 was used as the cut-off for Orien16S.

^bThe infection for sample No. 28 was from South Korea and the rest were acquired in Chile.