REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
The antarctic strain CCCryo 231-06 (= UTEX EE21; CCMEE 391) of the cyanobacterium Nostoc sp.	Biobank CCCryo at Branch Bioanalytics and Bioprocesses of the Fraunhofer Institute for Cell Therapy and Immunology (IZI-BB), Potsdam, Germany	CCCryo 231-06
Chemicals, peptides, and recombinant proteins
Pluronic F127	Sigma Aldrich	Cat# P2443; CAS: 9003-11-6
PBS	Fisher Scientific	Cat# BP243820
PDMS	Momentive	RTV615
UltraPure™ 0.5M EDTA, pH 8.0	ThermoFisher	Cat# 15575020
Ready-Lyse™ Lysozyme Solution	Lucigen	Cat# R1804M
Dithiothreitol (DTT)	Bio-rad	Cat# 1610611
Low-EDTA TE (1X), pH 8.0	VWR	Cat# 10128-588
NEBNext® Multiplex Oligos for Illumina®	New England Biolabs	Cat# E7335S
SPRIselect Beads	Beckman Coulter	Cat# B23318
Deposited data
Genome sequence	This paper	NCBI, accession# PRJNA721463, the locus tag prefix is KBT16.
Experimental models: Organisms/strains
The antarctic strain CCCryo 231-06 (= UTEX EE21; CCMEE 391) of the cyanobacterium Nostoc sp.	Biobank CCCryo at Branch Bioanalytics and Bioprocesses of the Fraunhofer Institute for Cell Therapy and Immunology (IZI-BB), Potsdam, Germany	CCCryo 231-06
Software and algorithms
Samtools	Schneider et al. (2012)	http://samtools.sourceforge.net/
BioBloomTools	Chu et al. (2014)	https://mybiosoftware.com/tag/biobloomtools
Atropos	Didion et al. (2017)	https://bio.tools/atropos
BBTools	https://sourceforge.net/projects/bbmap/, 2019	https://sourceforge.net/projects/bbmap/
MEGAHIT	Li et al. (2015)	https://bio.tools/megahit
BusyBee	Laczny et al. (2017)	https://ccb-microbe.cs.uni-saarland.de/busybee
CheckM	Parks et al. (2015)	https://github.com/Ecogenomics/CheckM
GTDBtk tool	Parks et al. (2018)	https://github.com/Ecogenomics/GTDBTk
Prokka	Seemann et al., 2014	https://github.com/tseemann/prokka
eggNOG mapper	Huerta-Cepas al., 2017	https://github.com/eggnogdb/eggnog-mapper
PATRIC	Davis et al. (2020)	https://www.patricbrc.org/
RAxML	Stamatakis et al., 2014	https://cme.h-its.org/exelixis/web/software/raxml/
Dendroscope 3	Huson et al., 2012	https://uni-tuebingen.de/
PHASTER	Arndt et al. (2016)	https://phaster.ca/
Other
REPLI-g Single Cell Kit	Qiagen	Cat# 150,343
NEBNext Ultra II DNA Library Prep Kit for Illumina	New England Biolabs	Cat# E7103
HiSeq 3000/4000 PE Cluster Kit	Illumina	Cat# PE-410-1001
HiSeq 3000/4000 SBS Kit (300 cycles)	Illumina	Cat# FC-410-1003