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. 2022 Apr 28;28:1610230. doi: 10.3389/pore.2022.1610230

FIGURE 2.

FIGURE 2

Representative immunostaining of CXCL13 and CD163 in human astrocytoma. Immunoreactivity of CXCL13 (A,B) and CD163 (C,D) was classified as low or high expression according to staining observed in cytoplasm and plasma membrane. (E,F) Double-staining of CXCL13 protein in tumor tissues (red cytoplasmic staining) and CD163+ M2 (brown plasma membrane staining) demonstrated co-occurrence of CXCL13 and CD163 in astrocytoma tissues. Black arrows indicate the CD163+ M2 macrophages infiltrate in malignant tissues (×200 magnification, scale bar 100 μm). Immunoreactivity percentages of CXCL13 (G), CD163 (H), and CXCL13/CD163 (I) phenotypes were observed in lesions. LGG subgroup is depicted as light gray columns, whereas GBM subgroup is shown as dark gray columns. Chi-square test was used for statistical analysis. *p < 0.05 was considered statistically significant. Abbreviations: LGG, lower grade glioma; GBM, glioblastoma multiforme; T, tumor tissue; NV, neovasculature.