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. 2022 May 11;13:2585. doi: 10.1038/s41467-022-30349-w

Fig. 6. L5 IT neurons modulate superficial layers more robustly than PT neurons.

Fig. 6

a Schematics showing potential feedback circuits via IT and PT neurons in L5. b Viral strategies for gene expression selectively in IT (top) or PT(bottom) neurons. c A1 coronal sections from mice expressing synaptophysin-EYFP in IT (left) or PT (middle) neurons. Right, distribution of EYFP signal intensity across the normalized cortical depth (IT, n = 3 mice, 5 sections; PT, n = 4 mice, 5 sections). Solid lines and shadings show mean and SEM. Black dotted line shows the mean of Rbp4-Cre data from Fig. 1b. d–g Data for activation of IT or PT neurons with ChrimsonR. d Left, raster plot and PSTH of spontaneous firing with (black) or without (red) LED in a representative photoactivated IT single-unit. Right, similar plots for a representative PT single-unit. e Laminar profile of MI for the spontaneous firing of RS multi-units during activation of IT (left) or PT (right) neurons. Individual data points are overlaid (IT, n = 5 mice; PT, n = 6 mice). Depth is normalized in each mouse according to the surface, L4 borders, and white matter positions. Red shades indicate L5a (left) and L5b (right), respectively. Results are mean ± SEM. f Scatter plots showing MI for the spontaneous firing of RS single-units in each layer across mice (IT: n = 33, 33, 36 units; PT: n = 37, 31, 43 units for L2/3, L4, L6). Red dots and bars represent median and 25th and 75th percentiles, respectively. *p < 0.05, **p < 0.01, ***p < 0.001. Individual groups: two-sided Wilcoxon signed rank test with Bonferroni correction (black asterisks). Comparisons across groups: two-sided Wilcoxon rank sum test with Bonferroni correction (brown asterisks). g Same as f for tone-evoked firing (IT: n = 11, 12, 11 tone-responsive units and n = 25, 27, 52 unit-frequency pairs for L2/3, L4, L6; PT: n = 15, 17, 19 tone-responsive units and n = 41, 48, 65 unit-frequency pairs for L2/3, L4, L6). h–k Same as in d–g but for inactivation of IT or PT neurons with eNpHR3. i IT: n = 5 mice; PT: n = 5 mice. j IT: n = 27, 37, 49 units for L2/3, L4, L6; PT: n = 28, 26, 32 units for L2/3, L4, L6. k IT: n = 10, 13, 28 tone-responsive units and n = 23, 20, 79 unit-frequency pairs for L2/3, L4, L6; PT: n = 16, 6, 11 tone-responsive units and n = 37, 12, 32 unit-frequency pairs for L2/3, L4, L6. See Supplementary Data 1 for additional statistics. Source data are provided as a Source Data file.