Figure 1.

HMGB1 reduced evidence for myelination after LPC-induced demyelination. (A) A representative IHC image of mouse spinal cord injected with 1 μl LPC (1.0%) and water control 10 days post lesion (dpl) stained with Olig2, CC1, and DAPI. (B) A representative IHC image of mouse spinal cord co-injected with 1 μl LPC (1.0%) and 1 μl rhHMGB1 (1 mg/ml in water) at 10 dpl stained with Olig2, CC1, and DAPI. (C) A representative IHC image of mouse spinal cord injected with PBS and 1 μl rhHMGB1 (1 mg/ml in water) at 10 days post injection stained with Olig2, CC1, and DAPI. (D) Quantification of the number of Olig2+ cells. (E) Quantification of the Olig2+ and CC1+ cells. (F) Percent of Olig2+ cells that were also CC1+. (G) Representative image of mouse spinal cord injected with 1 μl LPC (1.0%) and water control 10 dpl stained for myelin basic protein (MBP) and DAPI. (H) A representative IHC image of mouse spinal cord co-injected with LPC and rhHMGB1 at 10 dpl stained for myelin basic protein (MBP) and DAPI. (I) A representative IHC image of mouse spinal cord injected with PBS and 1 μl rhHMGB1 (1 mg/ml in water) at 10 days post injection stained for myelin basic protein (MBP) and DAPI. (J) Percent of the lesion area that was MBP+. (K) Quantification of the area of the lesion in mm². White dashed lines indicate lesion/injection site. Two to six spinal cord sections from each mouse were used for quantification, 3–5 mice per condition. ****P ≤ 0.0001; ***P ≤ 0.001; **P ≤ 0.01; and *P ≤ 0.05. ns when P > 0.05. Scale bar = 50 μm.