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. 2022 Apr 28;16:833186. doi: 10.3389/fncel.2022.833186

Figure 4.

Figure 4

HMGB1 inhibited OPC differentiation via TLR2/1 receptors. (A) Differentiation of rat OPC cultures after 72 h of treatment with differentation media, differentiation media plus HMGB1 or differentiation media plus HMGB1 and CU CPT22. (B) Quantification of the amount of differentiation, as measured by the percent of MBP+ cells, normalized to differentiation media using each receptor inhibitor [Not shown: all conditions are significantly different from HMGB1 (p < 0.0001), except FPS-Zm1 3 μM and 6 μM (ns)]. Analyzed by one-way ANOVA, where ****P ≤ 0.0001; ***P ≤ 0.001; **P ≤ 0.01; and *P ≤ 0.05. ns when P > 0.05. Scale bar = 100 μm.