Figure 6.

Gene ontology enrichment analysis of HMGB1 regulated NFκB gene pathways. Analysis of HMGB1 regulated transcriptional responses in OPCs identified differentially expressed NFκB target genes which reflected both induced (A) and repressed (B) patterns of gene expression. (C) GO analysis of repressed cellular processes in OPCs treated with HMGB1 as defined by NFκB-regulated transcriptional targets. No pathways in this analysis other than those listed had significant differences from control. (D) GO analysis of processes induced by HMGB1 identified biological pathways which emphasized cellular periphery as a major influence of HMGB1 on OPC activity. These data confirm the pathophysiological actions of HMGB1 in vitro and in vivo and provide a template for understanding of how HMGB1 potently affects OPC differentiation potential. P values depicted in (C and D) are indicated by color and the number of genes in each pathway category are indicated by a bar graph on the X axis for each panel.