FIGURE 4.
High pressure liquid chromatography (HPLC) spectra of Picochlorum sp. grown at (A) white, (B) blue, (C) green, and (D) red light, harvested at late exponential phase, at 150 μmol m−2 s−1 irradiation level. Peaks: 1 all-trans neoxanthin, 2 violaxanthin, 3 lutein, 4 canthaxanthin, 5 Chl b, 6 Chl a, 7 β-carotene, X1, X2, X3 and X4 unknown pigments (only occurring with green light). Measured on Kinetex 5 µm C8 column (150 mm * 4.6 mm), detection at λ = 450 nm. Known substances were identified by comparison to commercial standards.