TABLE 4.
Identification of chlorophylls by high resolution high pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis of chlorophyll (Chl) a and b and unknown pigments X1-X4.
| Pigment | Simulated matching MH+ ion formula | Simulated matching MH+ ion [m/z] | Actual MH+ ion [m/z] | Conformity value [ppm] | Potential construct |
|---|---|---|---|---|---|
| Chl a | C55H73O5N4Mg | 893.54259 | 893.54219 | -0.44315 | Chlorophyll a |
| X4 | C55H71O5N4Mg | 891.52694 | 891.52644 | -0.55846 | Chl a + 1 DB |
| X3 | C55H67O5N4Mg | 887.49564 | 887.49532 | -0.35664 | Chl a + 3 DB |
| Chl b | C55H71O6N4Mg | 907.52185 | 907.52182 | -0.04237 | Chlorophyll b |
| X2 | C55H69O6N4Mg | 905.50620 | 905.50598 | -0.24781 | Chl b + 1 DB |
| X1 | C55H65O6N4Mg | 901.47490 | 901.47479 | -0.12793 | Chl b + 3 DB |