Figure 5.

HSP27 overexpression attenuated neurological deficit and cell apoptosis after SAH in rats. (A) Schematic of AAV vector encoding HSP27 and experimental design. Representative images of rat brain on day 2 after surgery for the sham, SAH + vehicle, SAH + AAV vector (SAH + Con), and SAH + AAV vector encoding HSP27 (SAH + HSP27) group. (B) Behavior scores of each group were assessed at 48 h, n = 6. (C,D) Coronal sections from the sham (n = 6), SAH + vehicle (n = 6), SAH + Con (n = 6), and SAH + HSP27 (n = 7) group reperfusion on day 2 after SAH, subjected to immunostaining for the HSP27 (red) or TUNEL (green) in the basal cortex. Quantification was performed by counting the HSP27 or TUNEL positive cells per mm² region in the basal cortex, scale bar = 50 μm. Data are mean ± SD, *p < 0.05, ANOVA with Bonferroni's multiple comparisons test.