TABLE 1.
Key resources table.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Biological samples | ||
| Punch skin biopsy of non-diseased skin, removed during skin surgery of patient with breast ptosis and cutaneous squamous cell carcinoma | University Hospital Zurich | N/A |
| Punch skin biopsies from the patients with systemic sclerosis | University Hospital Zurich | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Dulbecco’s Phosphate Buffered Saline | Thermo Fisher Scientific | Cat#14190144 |
| RPMI 1640 medium, HEPES | Thermo Fisher Scientific | Cat#52400025 |
| Dispase II | Roche | Cat#04942078001 |
| DNase I | Roche | Cat#11284932001 |
| Collagenase IV | Worthington | Cat#LS004189 |
| Fetal Bovine Serum | Corning | Cat#35-079-CV |
| UltraPure™ DNase/RNase-free distilled water | Thermo Fisher Scientific | Cat#10977035 |
| UltraPure™ BSA (50 mg/ml) | Thermo Fisher Scientific | Cat#AM2616 |
| Trypsin-EDTA (0.25%), phenol red, 100 ml | Thermo Fisher Scientific | Cat#25200056 |
| Chromium Controller & Next GEM accessory kit | 10x Genomics | Cat#1000204 |
| Chromium Next GEM Single Cell 3′ GEM, library & gel bead kit v3.1 | 10x Genomics | Cat#1000121 |
| Chromium Next GEM Chip G single cell kit | 10x Genomics | Cat#1000120 |
| Single Index Kit T Set A | 10x Genomics | Cat#1000213 |
| 6-well cell culture plate | TPP | Cat#92006 |
| Cell strainer (70 µm) | Corning | Cat#352350 |
| ART™ wide bore filtered pipette tips | Thermo Fisher Scientific | Cat#2069G |
| Tissue culture dish | TPP | Cat#93100 |
| Scalpel | B. Braun | Cat#5518067 |
| 15 ml centrifuge tubes | TPP | Cat#91015 |
| Biopsy punch KAI 12 with plunger 20 pieces (4 mm) | Kai Medical | Cat#BPP-40F |
| 3 piece syringes (1 ml) | BD Medical | Cat#303172 |
| Cell strainer (40 µm) | Corning | Cat#352340 |
| 5 ml Round Bottom Polystyrene Test Tube, with Cell Strainer Snap Cap | Falcon® | Cat#352235 |
| Luna Automated Cell Counter counter + Photon Slides | Logos Biosystems Inc. | Cat#L1001+ L12005 |
| Acridine Orange/Propidium Iodide Stain | Logos Biosystems Inc. | Cat#F23001 |
N/A: not applicable.