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. 2022 Apr;14(4):1193–1202. doi: 10.21037/jtd-22-282

Figure 2.

Figure 2

TCS induces pyroptosis of A549 cells. (A) Immunofluorescence determines the effect of different concentrations of TCS (0, 10, 20, and 40 µg/mL) on the expression of GSDMD-N. (B) Immunofluorescence determines the effect of different concentrations of TCS (0, 10, 20, and 40 µg/mL) on the ROS levels. (C) Western blotting measures the effect of different concentrations of TCS (0, 10, 20, and 40 µg/mL) on the pyroptosis-related proteins (NLRP3, ASC, caspase-1, pro caspase-1, GSDMD-N and GAPDH). *P<0.05, **P<0.01, and ***P<0.001 vs. 0 µg/mL. GSDMD, gasdermin-D; DAPI, 4',6-diamidino-2-phenylindole; NLRP3, NLR family pyrin domain containing 3; ASC, apoptosis-associated speck-like protein containing a CARD; TCS, trichosanthin; ROS, reactive oxygen species.