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. 2022 May 12;12:7808. doi: 10.1038/s41598-022-11748-x

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© The Author(s) 2022

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No effect of BCG vaccination on growth of Gram-positive bacteria in the PBMC GIA. Samples were

taken from volunteers enrolled into phases 1 and 2 combined. Healthy UK adults were randomised to receive BCG vaccination (n = 27) or to be unvaccinated controls (n = 8). PBMC bacterial GIAs were conducted on samples taken at baseline and days 2, 7, 14, 28 and 84 following BCG vaccination (closed circles) and at the same time-points in unvaccinated control individuals (open circles). PBMC and autologous serum were co-cultured with S. aureus (a, b) or S. agalactiae (c, d) for 1 h after which time cells were lysed and bacteria quantified by plating on solid blood agar. Bars represent the median values with the interquartile range (IQR); dotted lines indicate the baseline median.