Figure 1.

General experimental design. (A) The MS protocol started on day one and finished on day 60; the n-3 supplementation protocol started on day 30 and ended on day 60; the migraine induction lasted from days 45 to 60. The animals were assessed in behavioral tasks and submitted to euthanasia for sample collection. (B) The chronic migraine protocol comprised five i.p. injections of NTG at days 47, 50, 53, 56, and 59; control animals received vehicle at the same treatment schedule. (C) Scheme showing the group distribution. Rats were initially distributed into two experimental groups that received tap water or 10% fructose solution for 60 days. After 30 days of supplementation onset, animals were randomly subdivided into control or n-3-treated groups. Additional subgroups were formed after 15 days of treatment: one-half of animals were subjected to the protocol of migraine induction, by five NTG i.p. injections every three days, whereas control animals received vehicle by the same route of administration. MS, metabolic syndrome; NTG, nitroglycerin; HFD, high fructose diet.