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. 2022 Apr;10(8):435. doi: 10.21037/atm-21-5699

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2022 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Effects of NTG, HFD, and n-3 treatment on hypothalamic levels of (A) CGRP, (B) IL-6, (C) TNF, (D) leptin, and (E) adiponectin, measured by enzyme immunoassay at the end of experiments. Data are presented as means ± SEM of 4 to 6 rats per group (one-way ANOVA followed by uncorrected Fisher’s LSD’s post hoc test). ^a, P<0.05 water + saline vs. HFD + saline; ^d, P<0.05 water + n-3 vs. water + n-3 + NTG; ^e, P<0.05 water + NTG vs. water + n-3+ NTG; ^f, P<0.05 HFD + saline vs. HFD + NTG. TNF, tumor necrosis factor; NTG, nitroglycerin; HFD, high fructose diet; SEM, standard error of the mean.