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. 2022 Apr;10(8):485. doi: 10.21037/atm-22-1298

Figure 1.

Figure 1

BBR inhibited proliferation and induced apoptosis in NSCLC cells. (A) The A549 and PC9 cells were treated with various concentrations of BBR for 24, 48, and 72 h. Cell viability was analyzed by CCK-8 assays. (B) Morphological changes were assessed via microscopy after a 48-h treatment (20×). (C) The A549 and PC9 cells were treated with a range of BBR concentrations (0, 40, and 80 μM) for 48 h, after which Annexin V/PI staining was used to evaluate apoptotic death by flow cytometry. (D) The apoptotic rates were quantified. (E) After the same treatment described in (C), CL-caspase-3, Bax, and Bcl-2 in the NSCLC cells were measured by western blotting. (F) The protein levels from (E) were quantified. The data are presented as the mean ± standard deviation for the three different experiments with triplicate sets in each assay. *P<0.05, ***P<0.001, and nsP>0.05. BBR, berberine; NSCLC, non-small cell lung cancer; CCK-8, Cell Counting Kit-8.