FIG. 2.

Immunostained cross blots showing the binding of H-specific antibodies to recombinant flagellin expression products. Binding of the H:i (A) and H:1,2 (B) flagellins with H-specific MAbs and commercial serotyping sera. Binding of the MAbs is indicated by the bar (filled, strong; hatched, weak; open, negative). Restriction sites used for the creation of fragments are indicated on the top bar of each panel, which represents the complete flagellin protein. The positions of the primers used for the cloning of the flagellin genes (E1, E2, P1, and P2) are depicted by arrows. Fragments indicated by superscript a were also cloned in pGEX and applied in an ELISA. Both a large fragment of the heterologous flagellin and cro-β-galactosidase (cro-β-gal) expressed from the empty vector pEX13 (indicated by superscript b) were always included as negative control fragments.