TABLE 1.
Validation of H antigen-specific MAbs compared to commercial serotyping antisera (Murex) with whole-protein cell extracts of various Salmonella serotypes in Western blots
| Common H antigen | Salmonella strain (serotype) | H:i-specific antibody bindingd
|
H:1,2-specific antibody bindingd
|
||||
|---|---|---|---|---|---|---|---|
| Serotyping antisera
|
MAbs | Serotyping antisera
|
MAbs | ||||
| 1b | 2b | 1c | 2c | ||||
| H:i:1,2 | S. typhimurium (i:1,2) human isolatesa | + | + | + | + | + | + |
| S. typhimurium (i) human isolatesa | + | + | + | + | + | + | |
| S. typhimurium (1,2) human isolatesa | ± | + | ± | + | + | + | |
| S. typhimurium (1,2) human isolatesa | − | − | − | + | + | + | |
| S. typhimurium (i) ATCC 14028a | + | + | + | ± | − | − | |
| S. aberdeena (i) | + | + | + | ± | ± | − | |
| H:i | S. bonariensis (i:enx) | + | + | + | − | − | − |
| S. kentucky (i:z6) | + | + | + | ± | ± | − | |
| S. bergen, S. jukestown (i:enz15) | + | + | + | − | ± | − | |
| S. takoradi (i:1,5) | + | + | + | ± | − | − | |
| S. bandia, S. kedougou (i:lw) | + | + | + | ± | − | − | |
| S. schalkwijk (i:en) | + | + | + | ± | ± | − | |
| H:1,2 | S. heidelberg, S. virchow (r:1,2) | ± | ± | − | + | + | + |
| S. muenchen (d:1,2) | ± | ± | − | + | ± | ± | |
| S. newport (eh:1,2) | ± | ± | − | + | + | + | |
| H:gm | S. enteritidis (gm:−) | − | − | − | − | − | − |
a
The dominant H antigen, determined by agglutination, is shown in parentheses.
b
Serum batch 1, lot no. K002510; serum batch 2, lot no. K973010.
c
Serum batch 1, lot no. 846810; serum batch 2, lot no. K506610.
d
Antibody binding by Western blotting: +, strong; ±, weak; −, negative.