Figure 8.

Effects of SKF96365 and 2-APB on [Ca²⁺] i in neutrophils. The neutrophils were pretreated under Hank’s balanced salt solution with 40 µΜ SKF96365, or with 100 µΜ 2-APB for 30 minutes. (A) The pretreated cells were suspended in Ca²⁺-free medium and stimulated with TG (2 µΜ) followed by the addition of 2 mM of extracellular Ca²⁺. The cells pretreated with SKF96365 or 2-APB showed a significant reduction in TG-induced Ca²⁺ influx. **, P<0.01, by a 1-way ANOVA. (D) The pretreated cells were suspended in Ca²⁺-free medium and stimulated with CSE followed by the addition of 2 mM of extracellular Ca²⁺. The cells pretreated with SKF96365 or 2-APB showed a significant inhibition of CSE-induced Ca²⁺ influx without a change in Ca²⁺ store depletion. (B,C,E,F) shows the quantification of Ca²⁺ at 500 s or 800 s during Ca²⁺ influx when stimulated by TG and CSE respectively. **, P<0.01, by a 1-way ANOVA. The figures on the right show the means ± SD from 3 independent experiments. TG, thapsigargin; CSE, cigarette smoke extract.