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. 2022 Apr 28;12:875539. doi: 10.3389/fcimb.2022.875539

Figure 5.

Figure 5

Analysis of the immunogenicity and vaccine efficacy of the rZH548-L mutant viruses in 129 (wt) mice. Groups of 4-month-old mice (n = 6, including male and female equally distributed) were inoculated with 500 pfu of rA2 and rB3 viruses. C1 (rZH548) and 2B7 (rZH548-P82L) were included for a comparison. 3 weeks later survivors were challenged with a lethal dose of 1000 pfu of C1 (rZH548). (A) Survival rates after first inoculation. (B) Percentage weight variation, geometric mean per group. The area between 97.5 and 102.5% has been shaded. (C) Viremia determined by RT-qPCR on EDTA blood samples collected at day 3 pi. Samples giving a Cq (quantification cycle) value under the detection level of the assay (37) are arbitrarily represented as 45. The correlation of Cq data with pfu equivalents (Moreno et al., 2020) is indicated in the right Y axis. (D) Neutralizing antibodies at day 12 pi as determined by a plaque reduction assay. (E) Viremia determined by RT-qPCR on EDTA blood samples collected at day 3after challenge. (F) Antibodies to N protein by ELISA both pre- and post-challenge (small and large symbols respectively).