Table 4.

Amino acid (one-letter code) found in the three positions of the L-protein under study (924, 1050 and 1303) as deduced from the corresponding nucleotide sequence.

		Aa 924	Aa 1050	Aa 1303
Reference viruses	rZH548 wt	G	I	A
	rZH548_Lmut inoculum	S	I	T
Viral RNAs recovered from IFNAR KO mice inoculated with rZH548_Lmut (rA2/rB3)	A1	S/G	I	T
	A2	S/G	I	T
	A3	S	I	T
	A4	S	I	T
	A5	S/G	I	T
	A6	S	I	T
	A7	G	V	T/A
	A8	S	I	T
	A9	S	I	T
	A10	S	I	T
	B1	S	I	T
	B2	S	I	T
	B3	G	V	A
	B4	G	V	A
	B5	G	V	A
	RVFV 40Fp8 mutant	S	V	T

For positions showing a nucleotide mix that led to a change in the corresponding codon, the two possible amino acids are indicated, with slash. Samples recovered are coded as A (animals inoculated with rRVFV_A2) or B (animals inoculated with rRVFV_B3). Each sample correspond to an individual mouse. Numbers 1 to 5 correspond to animals receiving the high dose of virus, 500 pfu; numbers 6 to 10, those inoculated with the low dose, 10 pfu (only for A2 group).