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. 2022 Apr 28;10:866491. doi: 10.3389/fcell.2022.866491

FIGURE 11.

FIGURE 11

Loss-of-function analysis of the seven newly discovered miRNAs. (A) Quantification of mRNA expression of hh, ptc, smo, cos2, fu, Su(fu), ci, ihog, boi, dally, dlp, col, and dpp in seven miRNA knockout mutant larvae by qPCR. Bar plots represent relative mRNA levels of indicated genotypes (n = 3); error bars represent standard deviation (S.D.). (B–E) The GFP expression of the gfp: 3’UTR fu sensor was significantly increased in miR-10 KO homozygotes (C) compared with miR-10 KO heterozygotes (B). The same goes for the gfp:3’UTR smo sensor in miR-958 KO homozygotes (D,E). (F–L) Adult wings of the indicated genotypes are shown. The distance between L3-L4 veins (green line in F), the distance between L2-L5 veins (blue line in F), and the size of adult wings (yellow area in F) were measured, (M,N) Shown is statistical analysis of the ratio of L3-L4 distance to L2-L5 distance (M), and wing size (N) for the indicated genotypes. Data are presented as mean ± S.D. (n = 20). One-way ANOVA followed by Dunnett’s tests was used. NS, not significant. *p < 0.05. **p < 0.01. ***p < 0.001. Scale bar, (B–E), 50 μm; F-L, 100 μm.