Skip to main content
. Author manuscript; available in PMC: 2023 Mar 9.
Published in final edited form as: Cell Host Microbe. 2022 Mar 2;30(3):314–328.e11. doi: 10.1016/j.chom.2022.02.001

Figure 3. B. uniformis contains a mobile plasmid that confers agarose utilization.

Figure 3.

(A) Circular map of the B. uniformis strain NBRC 113350 plasmid pBUN1 that contains an agarose utilization PUL. Strain designations for B. uniformis isolates in this study are located at the bottom. Sequence reads from each of the additional isolates in this study were mapped based on a minimum of 97% nucleotide identity to this scaffold, demonstrating that B. uniformis and B. xylanisolvens strains contain most of the episome. (B) Total growths on glucose, agarose and porphyran by B. uniformis plasmid donors (JM204–1P and 26A), B. vulgatus ATCC 8482 type strain and recipient B. vulgatus strains after mating with the indicated B. uniformis donor (Bv 8482 JM204–1P and 26A mated) showing acquisition of agarose degrading phenotypes. Error bars indicate standard deviation (SD) of the mean. Three replicate experiments were conducted for each treatment condition.