Table 3. SMFS-Derived Unbinding Force Values and Single Base Mismatch Discrimination Capacities as Demonstrated by the DNA and the XNA Probes.

	Mean unbinding force (pN)
Nucleic acid duplexes	Full match	Single base mismatch	Mismatch discrimination (pN)
DNA–DNA	124 ± 3	105 ± 2	∼19
PNA–DNA	95 ± 2	64 ± 1	∼31
MO–DNA	157 ± 2	108 ± 1	∼49
LNA–DNA	203 ± 2	123 ± 2	∼80