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. 2022 Apr 28;13:840397. doi: 10.3389/fpls.2022.840397

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Copyright © 2022 Li, Zhang, Zhao, Zhao, Xie, Guo, Wang, Li, Guo, Zhu, Zhang, Jia and Miao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The phylogenetic analysis and tempo-spatial expression pattern of the CAD genes in G. hirsutum. (A) Phylogenetic tree of CAD proteins from Arabidopsis and G. hirsutum. The phylogenetic tree was generated by the Neighbor-Joining (NJ) method with 1,000 bootstrap replicates. CAD proteins were divided into I-V groups distinguished by different colors. The nine bona fide CADs of Arabidopsis were marked by “⬤”; the nine candidate CADs of Arabidopsis identified in this study were marked by “★.” (B) The tempo-spatial expression pattern of 28 GhCAD genes. The expression of different GhCADs in the leaf, stem, and root tissues of 30-, 60-, 90-day old plants, respectively. Three biological replicates were performed for each treatment. The expression of GhCADs was relative to that of GhUBQ7.