FIGURE 2.

V. dahliae inoculation induces the expression of several GhCADs in different G. hirsutum cultivars. (A,B) The transcriptional levels of two pathogen defense-related genes PR10-5 and PR10-11 under control (ddH₂O) and V. dahliae treatment conditions in different G. hirsutum cultivars. (C–E) The transcriptional levels of GhCAD35, GhCAD45, GhCAD43, and GhCAD40 under control (ddH₂O) and V. dahliae treatment conditions in different G. hirsutum cultivars. Four Verticillium wilt resistant cultivars (ZZM 2, Han 333, GK 44, and GK168), four Verticillium wilt susceptive cultivars (Jimian 11, DH 966, Foster 6, and 8891) and TM-1 were used in the V. dahliae inoculation experiment; two-weeks-old plants 18 days after V. dahliae inoculation were used for qRT-PCR analysis. Three biological replicates were performed for each treatment. The gene expression level of each gene in TM-1 under the control (ddH₂O) treatment was normalized as 1. GhUBQ7 was used as reference gene.