FIGURE 6.

Quantitative analyses of H-, G-, and S-units of lignin in the stem tissues from TRV:00, TRV:CAD35, TRV:CAD45, and TRV:CAD43 VIGS plants under the control (ddH₂O) and V. dahliae treatment conditions. (A–C) Quantitative analyses of H-, G-, and S-units of lignin in TRV:00, TRV:CAD35, TRV:CAD45, and TRV:CAD43 VIGS plants, respectively. The y axel on the left indicates the contents of individual unit; the y axel on the right indicates the relative content of individual unit, TRV:CAD35, TRV:CAD45, and TRV:CAD43 to that of TRV:00. The number on top of the gray bars indicates the increase percentage of each unit under V. dahliae infection relative to the control (ddH₂O); *P < 0.05, **P < 0.01 (Student’s t-test). The different letters above the black bars indicate significant differences; P < 0.05 (Student’s t-test). (D) The relative cell wall residue (CWR) in TRV:00, TRV:CAD35, TRV:CAD45, and TRV:CAD43 VIGS plants under the control (ddH₂O) and V. dahliae treatment conditions. The number on top of the Ebars indicates the increase percentage of each unit under V. dahliae infection relative to the control (ddH₂O); *P < 0.05 (Student’s t-test); the data represent the mean ± SD (n = 3 biological replicates). Two-weeks-old VIGS-plants were inoculated with V. dahliae at an inoculum of 1 × 10⁷ spores/mL using the root-dipping method. The samples were collected 48 h after the inoculation.