FIGURE 7.

Histochemical analyses of lignin in the stem cross-sections from TRV:00, TRV:CAD35, TRV:CAD45, and TRV:CAD43 VIGS plants at 18 dpi. (A) The handcut stems were illuminated by UV or stained with phloroglucinol-HCl to detect lignin. (B) Close-up of UV autofluorescence and phloroglucinol-HCl stain of stems corresponding to images in A. Two-weeks-old VIGS plants were inoculated with V. dahliae at an inoculum of 1 × 10⁷ spores/mL using the root-dipping method. Scale bars in A and B represent 1 mm and 100 μM, respectively. The white scales in V. dahliae inoculated stems under UV indicate the width of xylem; the red arrows indicate the vessels.