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. 2022 Apr 28;13:840397. doi: 10.3389/fpls.2022.840397

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Copyright © 2022 Li, Zhang, Zhao, Zhao, Xie, Guo, Wang, Li, Guo, Zhu, Zhang, Jia and Miao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing of GhCAD35, GhCAD45, and GhCAD43 impaired V. dahliae induced SA responses. (A) Quantification of SA contents in TRV:00, TRV:CAD35, TRV:CAD45, and TRV:CAD43 VIGS plants 8- and 12-h post-infection by V. dahliae. (B–E) The expression level of SA biosynthesis gene ICS and SA responsive genes PR1, PR5, and NPR1 in TRV:00, TRV:CAD35, TRV:CAD45, and TRV:CAD43 VIGS plants 12-h post-infection by V. dahliae. The data represent the mean ± SD (n = 3 biological replicates), *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test).