FIGURE 9.

Heterogeneously expressing of GhCAD35, GhCAD45, and GhCAD43 in Arabidopsis enhanced resistance to V. dahliae infection. (A) Phenotypes of the transgenic Arabidopsis plants overexpressing GhCAD35, GhCAD45, or GhCAD43 at 14 days post-infection (dpi) by V. dahliae. (B) Ratio of different diseased grades and disease index for GhCAD35, GhCAD45, and GhCAD43 overexpressing transgenic Arabidopsis plants at 14 dpi by V. dahliae. Two independent Arabidopsis transgenic lines of 35S:GhCAD35-GFP, 35S:GhCAD45-GFP, and 35S:GhCAD43-GFP were used for V. dahliae infection. 35S:GFP Arabidopsis transgenic plants were used as control. Two-weeks-old Arabidopsis transgenic plants were inoculated with V. dahliae via root-dipping method; 16 plants for each line were used. (C) The expression level of GhCAD35, GhCAD45, and GhCAD43 in the corresponding Arabidopsis transgenic plants determined by qRT-PCR.