Skip to main content
. Author manuscript; available in PMC: 2022 May 12.
Published in final edited form as: Dev Cell. 2021 Mar 15;56(6):811–825.e6. doi: 10.1016/j.devcel.2021.02.022

Figure 5. Rheb binding to PDPs enhances its activity to PDH.

Figure 5.

(A and B) GST pull-down (A) and quantification (B) comparing GST-Rheb pull-down of PDP2 and PDH-E1α from liver of Rheb KO versus mTor KO mice. Despite equivalent amounts of PDP2, less PDH-E1α is pulled down from Rheb KO. N = 3 independent experiments, PDP2, p = 0.8183; PDH-E1α, p = 0.0034.

(C) Subcellular fractionation showing comparable amounts of PDP2 and PDH-E1α in liver mitochondria of Ctrl and Rheb KO mice. Prohibition, the mitochondria marker; cyto, cytosol; and Mito, mitochondria.

(D) Incubation of Rheb protein with PDP1 increases the phosphatase activity of PDP1. N = 8 independent experiments.

(E) Diagram illustrating that Rheb activates PDH consequent to binding PDH phosphatases (PDP) and enhancing the association of PDH with PDP. All data represent mean ± SEM. Statistical analysis was performed by using two-tailed Student’s t test (B) or one-way ANOVA with Tukey post hoc test (D), and *p < 0.05, **p < 0.01, and ***p < 0.001. See also Figure S4.