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(A) Binding curve of TAMRA-JB006 to myotoxin II. FP saturation curve for the estimation of Kd between TAMRA-JB006 and myotoxin II, giving an estimated Kd value of 130 ± 30 μM. (B) FP increase in the absence of myotoxin II. Artificial increase in the FP signal in the absence of myotoxin II, but with increasing concentrations of non-labeled JB006-free peptide, indicates interactions between the JB006 peptide and the TAMRA probe. Results are presented as mean ± SD.
