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. 2022 Apr 25;7(18):15561–15569. doi: 10.1021/acsomega.2c00280

Figure 3.

Figure 3

Coomassie-stained SDS-PAGE images for myotoxin II pulldown experiments, depicting the section of the gel where myotoxin II migrates. (A) Myotoxin II and B. asper crude venom, reduced (red.) and non-reduced. (B) B. asper crude venom incubation at 4 °C overnight with streptavidin-agarose resin loaded with biotinylated JB006 peptide shows selective enrichment of myotoxin II. (C) B. asper crude venom incubation at 4 °C overnight with streptavidin-agarose resin loaded with biotin did not result in enrichment of myotoxin II. See Methods for details of the experimental protocols. The complete pictures of the gels are shown in Figure S2.