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. 2022 Apr 25;7(18):15561–15569. doi: 10.1021/acsomega.2c00280

Figure 4.

Figure 4

Inhibition of the cytotoxic activity of myotoxin II by different peptides (JB001–JB006). C2C12 myotubes were used. Myotoxin II (10 μg; 0.73 nmol; 7.3 μM) was pre-incubated for 30 min at 37 °C with 900 μM of each peptide in assay medium or without peptides and added in triplicate wells. After an incubation of 3 h at 37 °C, the activity of LDH released by damaged cells was measured on the supernatant. 100% release corresponds to cells incubated with 0.1% Triton X-100. Results are presented as mean ± SD (n = 3). Incubation of myotoxin II with JB006 caused a significant reduction of LDH release (4 ± 5%) compared to cells treated with myotoxin II alone (87 ± 11%). P < 0.05 (*), P < 0.01 (**), P < 0.001 (***), and P < 0.0001 (****).