Skip to main content
. 2022 Apr 28;13:821492. doi: 10.3389/fphar.2022.821492

FIGURE 1.

FIGURE 1

KMUP-1 attenuates osteoclast differentiation induced by PgLPS in RANKL-primed RAW264.7 cells through PKG pathway. (A) Scheme of experimental protocol for evaluation of KMUP-1 on PgLPS-induced osteoclast differentiation. RAW264.7 cells were pre-incubated with RANKL 1 day before PgLPS stimulation in the presence of various concentrations of KMUP-1 which was applied 1 h before PgLPS. KT5823 was applied 1 h before KMUP-1 when studying the effect of the cGMP-PKG pathway, otherwise RANKL-containing medium was replaced by KMUP-1-containing medium. (B) The representative images of TRAP stain with high power views on top row. Arrows indicate TRAP positive multinucleated cells (TRAP+ MNCs). (C) Statistical analysis of TRAP+ MNC number from (B). (D) TRAP activity assay. (E) Elevation of cGMP level by KMUP-1. (F) KT5823 reverses KMUP-1’s effect on suppression of osteoclast differentiation induced by PgLPS as demonstrated by TRAP stain. Scale bars: 100 μm. Data are the mean ± SD of three-independent experiments, with more than duplicate were performed in each experiment. #p < 0.05, ###p < 0.001 compared with control; *p < 0.05, **p < 0.01, ***p < 0.001 compared with PgLPS; ++p < 0.01 compared with PgLPS + KMUP-1.