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. 2022 May 12;20:223. doi: 10.1186/s12951-022-01442-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — In vitro chemo/chemodynamic therapy based on GCBD NPs. a Confocal laser scanning microscopy (CLSM) imaging of 4T1 cells stained with DCFH-DA after various treatments. Scale bar: 20 μm. b The GSSG/GSH ratio of GSH solutions (10 mM) after incubation with PBS, GCB NDs or GCBD NPs for 24 h. c The relative GSH content in 4T1 cells after incubation with different solutions for 24 h. d Schematic illustration showing the mechanism for cellular chemo/chemodynamic therapy based on GCBD NPs. P values in (b, c) were calculated by multiple t tests (****P < 0.0001, ***P < 0.001, ***P < 0.001, **P < 0.01, *P < 0.05)