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. 2022 Apr 29;7(18):15951–15968. doi: 10.1021/acsomega.2c01089

Figure 5.

Figure 5

Membrane permeabilization effect of P32 and P36. (a) Structural stability of calcein-entrapped LUVs (3:1 ratio POPE/POPG) in the absence of peptides (inset) monitored over a time period of 3600 s. Unchanged hydrodynamic radius of the LUVs after 24 h (green line) as seen from DLS. Absence of calcein leakage in the untreated LUVs. (b) Calcein dye leakage assay. Bar diagram representing calcein leakage from dye-entrapped LUVs (3:1 ratio of POPE: POPG), a microbial membrane mimic system, upon addition of increasing concentrations of P32 and P36. Triton X-100 (0.1%) was used as the positive control. Time kinetics of (c) NPN and (d) PI uptake after addition of 1× and 2× MIC P36 to P. aeruginosa cells, which indicate the outer and inner membrane permeabilities, respectively. All of the experiments were performed in triplicate.