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. 2021 Dec 13;220(2):iyab227. doi: 10.1093/genetics/iyab227

Table 1.

Data used for assembly of the PR1 genome

Sequence type Number of reads N50 read length (bp) Genome coverage
Illumina paired-end 1,307,137,030 150 59×
PacBio CLR 23,672,554 28,837 113×
PacBio HIFI 5,485,642 19,528 34×
ONT Ultralong 16,293,849 44,052 35×

N50 read length is defined as the length such that 50% of the total sequence is contained in reads of length N50 or longer. Note that the effective length of the Illumina reads was shorter after removing barcodes and adapter sequences.