Table 1.
Data used for assembly of the PR1 genome
Sequence type | Number of reads | N50 read length (bp) | Genome coverage |
---|---|---|---|
Illumina paired-end | 1,307,137,030 | 150 | 59× |
PacBio CLR | 23,672,554 | 28,837 | 113× |
PacBio HIFI | 5,485,642 | 19,528 | 34× |
ONT Ultralong | 16,293,849 | 44,052 | 35× |
N50 read length is defined as the length such that 50% of the total sequence is contained in reads of length N50 or longer. Note that the effective length of the Illumina reads was shorter after removing barcodes and adapter sequences.