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. 2021 Oct 12;220(2):iyab174. doi: 10.1093/genetics/iyab174

Figure 4.

Figure 4

AtTIR1(WT) allele of TIR1 generates ligand-independent loss-of-function phenotypes that are alleviated by the 5-Ph-IAA regulated AtTIR1(F79G) variant. (A) An AID-tagged, endogenous allele of ama-1, ama-1(ers49), exhibits a severe reduction in brood size when combined with AtTIR1(WT) even in the absence of added auxin. In contrast, the AtTIR1(F79G) variant does not alter the brood size of ama-1(ers49) animals (n = 15 for each genotype and significance was calculated using an unpaired t-test). (B) The expression of AMA-1::AID*::GFP is dramatically reduced in animals expressing AtTIR1(WT) and not in animals expressing AtTIR1(F79G). Shown are DIC and corresponding GFP images of vulval precursor cells (VPCs) (from early L3 stage animals at the P6.p 1-cell stage). The scale bar is 10 µ. (C) Quantification of AMA-1::AID*::GFP expression animals depicted in (B). Data presented as the mean ± SD (n ≥ 10 animals examined for each, and P < 0.0001 by a Student’s t-test). (D) AMA-1::AID*::GFP is degraded in the germline by 5-Ph-IAA. Representative DIC and GFP images of L4 staged animals that have been transferred to control NGM plates or NGM plates containing 0.5 mM 5-Ph-IAA for 2 h.