Table 1.

Measurement of TIR1 and TIR1(F79G) dependent heterochronic phenotypes in strains harboring an AID-tagged lin-28 allele

			Experimental	% Animals		% Animals		Ave. seam	Seam		% pvl
No.	Strain	Genotypea,b	Conditionc	L4 alaed	n	Adult alae	n	Numbere	Cell range	n	Animals	n
1	RG773	Wild type	—	0	20	100	20	15.8	15–17	22	0	100
2	HML1023	lin-28(ga54)I	—	85	20	100	15	11.8	10–12	27	100	100
3	ME462	lin-28::AID(ae157) I	—	0	20	100	20	16	15–17	26	0	100
4	HML1030	lin-28::AID(ae157) I; cshIs128[rps-28pro::TIR1_P2A_mCherryHis-11] II	—	86	21	100	20	12.68	12–16	25	68	100
			1 mM auxin	90	20	100	20	11.7	10–12	26	85	100
			1uM 5-Ph-IAA	85	20	100	20	12.24	11–12	25	100	100
5	HML1024	lin-28::AID(ae157) I; cshIs140[rps-28pro::TIR1(F79G)_P2A_mCherryHis-11] II	—	0	20	100	20	16	16–17	42	0	100
			1 mM auxin	0	20	100	20	15.8	15–16	31	0	100
			1uM 5-Ph-IAA	91	23	100	20	11.7	11–12	23	100	100

^aAnimals contain wIS78 V [ajm-1::GFP and scm::GFP] which were used to visualize adherence junctions and lateral seam cells.

^bL-the ae157 allele of lin-28 harbors an AID tag fused in frame to the lin-28 coding sequence generating a carboxy-terminal tagged allele.

^cL-4-staged Po animals were plated onto indicated plates and F1 progeny of the indicated stage were scored for.

^dPresence and quality of cuticular alae structures were assayed by Normarski DIC optics. Only one side of each animals was scored.

^eAverage seam cell numbers were scored by counting the number of SCM::GFP(+) cells on a single side of each animal.