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. 2021 Dec 9;220(2):iyab212. doi: 10.1093/genetics/iyab212

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© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pcr1-dependent control of the CCAAT and Oligo-C hotspots is not mediated by the recruitment of Pcr1. Chromatin immunoprecipitation of Pcr1 was used to test whether it is recruited to the test locus (ade6) by the CCAAT and Oligo-C hotspots in vegetative cells (mitosis) and cells in meiotic prophase (meiosis). Signal intensities from ChIP vs the input material used for ChIP (ChIP signal %) were compared to those of internal positive (hsp9) and negative (cdc18) controls. Data are mean ± SD from three independent biological replicates.